Objective To investigate the distribution patterns of mosquitoes and mosquito-borne viruses in northeast Guizhou province. Methods In 2017, host-seeking mosquitoes were collected from livestock sheds using Ultraviolet lights in Dejiang and Jiangkou, Guizhou province. The mosquito specimens were classified according to morphology and stored in liquid nitrogen. All the samples were incubated with BHK-21 and C6/36 cells for virus isolation and detected the virus genes. The virus isolates were identified and analyzed by molecular biological methods. Results In total, there were 7 380 mosquitoes from 3 genera and 3 species collected from Dejiang and Jiangkou, Guizhou province, 61.55% (4 542/7 380) of which were Armigeres subalbatus and 26.80% (1 978/7 380) were Anopheles sinensis. Out of the 3 virus strains isolated from the mosquitoes, One (GZDJ1765) was identified as typeⅠof Japanese encephalitis virus (JEV) isolated from Culex tritaeniorhynchus and two (GZDJ1746-1, GZDJ1752-1) were identified as Getah virus isolated from A. subalbatus. Three mosquito pools (GZDJ1704, GZDJ1743-2, GZDJ1751-2) tested positive for JEV genes y by PCR. Conclusion Armigeres subalbatus was the predominant species in northeast Guizhou. JEV and GETV were isolated.

This paper reports 2 genera, 16 species of hematophagous midges in 2016 in Dejiang county, Guizhou province, China, among which 13 species in Culicoides and 3 species in Lasiohelea. A new species C. ( Oecacta) qinglongensis Liu, sp. nov. was described. Five species, C. actoni, C. kongmiaoensis, C. newsteadi, C. parahumeralis, and La. danxinensis were recorded for the first time in Guizhou province. Culicoides ( O.) qinglongensis Liu, sp. nov. wassomewhat allied to C.( O.) okinawensis Arnaud, 1956, but was distinctly different from wing length 1.38 mm, antennasensilla coeloconica present on segments 3-14, antennal ratio (AR)1.28, mandible with 17 teeth of female; and shape of aedeagus, parameres, and basistyle with ventral root of male of C. ( O.) okinawensis Arnaud, 1956. The type specimens are deposited in the PLA Shenyang Command Center for Disease Control and Prevention (Shenyang 110034).

Objective In order to provide scientific basis for Brucellosis control and prevention, sero-epidemiological survey, Brucella isolation and genetic characteristic analysis were conducted for the high-risk population in a human Brucellosis epidemic in Weining county of Guizhou province. Methods Tube agglutination test was used to detect the anti-Brucella antibody for the high-risk human population. Rose Bengal Plate Test was applied to detect the antibody in goat blood samples. The blood of antibody positive human population was collected for Brucella isolation. Conventional methods, genus specific BCSP31-PCR and species-specific AMOS-PCR were used to identify the bacteria strain, and the genetic characteristic was analyzed using MLVA and MLST techniques. Results Six people of 43 high-risk populations were confirmed as anti-Brucella antibody positive, 64 out of 302 goat blood samples were anti-Brucella antibody positive, with positive rate of 21.19%. A suspected Brucella strain were isolated from one of the high-risk human populations and was identified as B. melitensis biovar 3. MLVA-16 analysis indicated that the bacteria strain was most closely clustered with B. melitensis biovar 3. Multilocus sequence typing analysis indicated the strain was ST8. Conclusion Genetic characteristic of Brucella strain isolated from the Brucelosis epidemic was consistent with that of M. melitensis biovar 3. Although antibody and Brucella were detected, the high-risk populations did not displayed symptoms, so all of them were asymptomatic infections. The epidemic was seemingly imported due to goats trading, suggested that health and animal disease control and prevention departments and doctors should pay great attention to it.

【Abstract】 Objective To investigate the influence factors in areas where rabies raged in Guizhou province, and provide the evidence for the establishment of focused intervention measure suitable for the rural areas. Methods A multistage sampling method was used in this study and all the data were analyzed by Excel 2003 after logging data with Epi Data. Results In recent years, the incidence of rabies in Zhenning county was very serious. The incidence rates of rabies in 2005 and 2006 were 6.16/10 thousand and 5.19/10 thousand respectively, which mainly caused by bite of dogs. Because of the large number of the dogs, the injury rate of the crowd was also higher. It was 6.47% in 2006, and there was significant difference between exposure rate of students （10.58%） and that of farmers （5.05%）（χ2＝36.57, P＜0.001）. About 31.40% exposed crowd were not done the medical treatment, and 35.41% exposed crowd without inoculation rabies vaccines, only 4.26% of Ⅲ° exposed crowd with rabies immunoglobulin. Villagers knew less about the knowledge of rabies prevention and control. Conclusion The main factors leading to the rage of rabies were large number of dogs, low immunization rate of the crowd, high exposure rate of the public and low treatment rate of exposed crowd in the investigated districts.

【Abstract】 Objective To study the influence of season, temperature and humidity to the seasonal fluctuation of Haemaphysalis bispinosa.  Methods Investigate 3 d in succession in the fixed sites per ten days monthly, the ticks were captured by the flag (90 cm×60 cm) at its active peak. The average quantity of ticks captured in successive 3 d per ten days was counted, and the temperature and humidity were recorded. The curve of average ticks number to time was drawn. Results The ticks captured belonged to H.bispinosa. Its density began to increase since April, and it had two population peak, one was in May and June, the other was in September and October.  After October, it decreased obviously, and its density was minimum in February and March. The activity peak of ticks was perhaps from May to June, and its propagation peak was from September to October. The curve of temperature change and the seasonal fluctuation of H.bispinosa was very similar, so the combination of humidity and temperature possibly affected the activity of H.bispinosa. Conclusion H.bispinosa could be active in the whole year, and the Summer and the Autumn were its activity peak.

Objective According to cases surveillance data of Japanese encephalitis(JE) from 2004 to 2005 in Guizhou province, understand the proportion of JE diagnosed cases to reported JE cases and master the epidemic status of JE to provide the science base for the control of JE case. Methods To analyze synthetically JE epidemic status based on epidemic situation surveillance management system, JE cases questionnaire, laboratory results and JE cases information of 2 surveillance counties. Results There were 1230 and 1038 cases in 2004 and 2005 respectively. The cases under 15 years old accounted for 96.58%(1188/1230) and 97.69% (1014/1038) respectively. The ratio of male to female was 1.58∶1 and 1.57∶1, respectively. The cases peak was in the 7-8 month, accounted for 78.21%(962/1230) and 79.09%(821/1038). The cases accounted for 88.36%(319/361) in two national surveillance counties. It was remarkable seasonal and highly dispersed for JE. The population with vaccine history was only 6.90%(26/377) and 8.86%(32/361). The positive rate of JE IgM antibody was 74.90%(188/251) and 71.74%(259/361) respectively. And it reached 71.88%(23/32) in surveillance counties. The JE cases reduced 15.61% in 2005 compared to that of 2004, and it decreased 39.47% in surveillance counties. Conclusion The diagnosed JE cases accounted for 71.88%-74.90% in Guizhou province. It should further strengthen JE surveillance system, enhance laboratory diagnosis technique so as to diagnize effectivly JE cases, and put the emphasis on the immunization of JE vaccine to the population of under 15 years old.